Antiwrinkle composition and age reversal complex

ABSTRACT

An alkaline anti-wrinkling cosmetic coposition comprising carnosine, derivative thereof or mixture of coanosine and derivative thereof.

[0001] This application claims the benefit of U.S. ProvisionalApplication No. 60/410,555, filed Aug. 8, 2002.

BACKGROUND OF THE INVENTION

[0002] This invention relates to a cosmetic composition containcarnosine that is highly effective in controlling the ageing process byreversing, preventing and reducing wrinkling of skin. In particular theinvention includes a complex containing carnosine that is especiallyeffective in reversing, preventing and reducing the aging process thatis visually observable through the formation of skin wrinkles. Duringthe aging process glycated end products of bonding between proteinmaterial and sugar molecules naturally leads to formation of deepwrinkles in the skin that are particularly visible in normally exposedareas such as the face. Without being bound to the reasons for theeffectiveness of the product of the present invention, it is theorizedthat carnosine prevents, reduces and can reverse the formation of suchglycated end products and also breaks the protein-sugar bonds to enableactual reversal of skin wrinkles. If glycosylation were not prevented itwould proceed as follows: a sugar/aldehyde molecules approach proteinmolecules (for instance, malondialdehyde could approach, attach to andinactivate healthy protein molecules); b. sugar molecules attach toprotein molecules leading to carbonylation crosslinking; c. the proteinsthat are carbonylated crosslinks (a detrimental factor); and d. advancedglycosylation end products (GRE's) form, which can cause extensivedamage by reacting with free radicals and other toxins so that AGE'sdeposit in the cell structure (it is noted in this regard thatmalondialdehyde is the result of free radical damage to lipids, thefatty portion of cells, leading to a vicious cycle of aging (observed byskin wrinkling) since oxidation results in free radical formation thatproduces malondialdehyde, causing glycosylation that results in AGE'sleading to more free radicals to repeat the process).

[0003] Prior research has determined that carnosine, with its knownanti-glycating properties, may provide an anti-aging effect onfibroblast material by inhibiting cross-linking of glycoxidized proteins(Hipkiss et al, Mech. Ageing Dev., Sep. 15, 2000, 122(13), p.1431-1445). Carnosine is reactive as an anti-glycating agent withaldehydes and ketones, that is compounds containing carbonyl groups(Hipkiss et al, Cell Mol. life Sci., May, 2000, 57 (5), p. 747-753capable of blocking carbonylated proteins, that form in step b.,describe above, and thus prevent the formation of detrimental AGE's.

[0004] Carnosine and complexes containing carnosine have been describedin skin care compositions and to promote wound healing, as in U.S. Pat.No. 5,091,171 to Yu et al; U.S. Pat. No. 5,723,482 to Degwert et al;U.S. Pat. No. 5,606,588 to Zaloga et al, and U.S. Pat. No. 4,981,846 toMatsukura et al. It is noteworthy, with particular regard to the patentto Yu et al, that while various cosmetic effects are disclosed forcompositions containing an alpha-hydroxyacid or alpha-ketoacid andvarious amphoteric materials, including dipeptides. It is specificallydisclosed that although the presence of alkali (ammonium hydroxide) doespermit the composition to be therapeutic for dry skin, it loses much ofits potency against wrinkles. Carnosine is disclosed among thedipeptides set forth. However, the patent does not recognize that itcould be effective against skin wrinkles under alkaline conditions.

[0005] It is an advantage of this invention that an alkaline skincosmetic composition is provided that is topically applied andtransdermally effective against wrinkling of skin.

[0006] It is a further advantage of this invention that the anti-wrinklecomposition contains carnosine, derivatives thereof or mixtures ofcarnosine and derivatives thereof

[0007] It is a further advantage of this invention that the carnosineand/or its derivatives are present in a complex that can be observed toreverse, prevent and reduce the aging process with regard to reversing,preventing and reducing formation of skin wrinkles.

[0008] Additional advantages resulting from the use of carnosine,including its derivatives include:

[0009] 1. Ability to combine with sugar aldehydes such asmalondialdehyde and inactivate them, thereby preventing carbonylationcrosslinking;

[0010] 2. As antiglycosylating agents they are superior in activityagainst free radicals to usual antioxidants, such as Vitamins E and Cand the like that are not antiglycosylating agents;

[0011] 3. They are antiglycosylating nutrients;

[0012] 4. They inactivate free radicals within the cell after theirformation;

[0013] 5. They increase elimination of materials already damaged by freeradicals;

[0014] 6. They are effective against chronic inflammation;

[0015] 7. They act as chelating agents to bind heavy metals andinactivate them, as well as assisting In regulating the concentration ofthese heavy metals (especially zinc and copper) to raise or lower theconcentrations to desired levels;

[0016] 8. They have antioxidant effect by stabilizing cell membranes andpreventing free radical damage especially to the cell membranes;

[0017] 9. They reduce or block excessive nitric oxide activity byblocking activation of guanylate cyclase, which is normally activated bynitric oxide.

[0018] It is a further advantage of this invention that the alkalinecomposition is compatible with other cosmetically desirable components,particularly non-allergenic components.

[0019] Additional advantages will be apparent from consideration of thefollowing specification

BRIEF DESCRIPTION OF THE INVENTION

[0020] In accordance with certain of certain of its aspects, thisinvention relates to an alkaline cosmetic composition having a pH ofabout 7.5 to about 9.5 that is effective against skin wrinkling, saidcomposition comprising carnosine, derivatives thereof and mixtures ofcarnosine and derivatives thereof dispersed in an alkaline cosmeticallyacceptable vehicle in effective antiwrinkle amount up to about 50% byweight of carnosine

DETAILED DESCRIPTION OF THE INVENTION

[0021] Carnosine is a naturally occurring dipeptide composed ofβ-alanine and histidine and has a molecular weight of 226.24. It iscommonly found in brain and innervated tissues and the ocular lens.Advantages obtained with the use of carnosine in the present inventionare not characteristics of p-alanine and histidine, even when use inmixture with each other. It may be employed in its α- and β-forms aswell as mixtures thereof. The α-form (also called L-carnosine) ispreferred and the most readily available. It is most preferably used inits uncomplexed form although it may in a complex salt or salts, forinstance with zinc, copper or manganese or in uncomplexed or suchcomplex salts of derivative compounds, such as N-acetylcarnosine, whichis a lipid soluble acid. When this lipid soluble acid is used, the pHrange of about 7.5 to about 9.5 is maintained with use of alkaline waterin the composition or by buffers. Other derivatives of carnosineincluded in the present invention are anserine (that is methylation ofcarnosine), homocarnosine, N-α-acetylcarnosine, carcinine and numerousvariants that are imidazole di-peptides. Such derivatives are describedin the book, Carnosine And Other Elixirs of Youth-The MiraculousAnti-Ageing Supplement, Kyriazis, 2003, Watkins Publishing, London.(Carnosine, a derivative thereof or mixture of carnosine and derivativethereof is employed in the cosmetic composition in an effectiveantiwrinkle amount typically about 0.5% to about 50% that is about 5mg/ml to about 500 mg/ml of composition), preferably about 1.5% to about30% and most preferably about 10% to about 20% by weight

[0022] Additional ingredients that cooperate with and are compatiblewith carnosine to achieve nutrient balance and improve anti-wrinklingeffectiveness include cascading antioxidants such as rosemarinic acid(that is, carnosac acid or caronsol embilica and additional polyphenolantioxidants, as well as anti-inflammatory materials such asbeta-glucan, alkyl alcohol amines and the like. In this regard, it isnoted that carnosine possesses antioxidant and anti-inflammatoryproperties.

[0023] It is also desirable to provide carnosine or its derivativeeither as such or as a complex salt and a complex composition thatcontains one or more of penetration enhancers of skin such asphosphotidyl, choline, serine and L-theanine, neutralizing agents forreactive radicals such as tocotreniols, especially in the gamma form,γ-tocophenol and mixed α-, β- and γ-tocotreniols, anti-inflammatoryagents such as glycerhizinate of licorice extract, cell stimulants suchas glutathione and kinetin, growth promoting and bio-activating agentssuch as L-tyrosine and N-acetylcystine, and enhancers of the immunesystem such as clostrum and lactoferrin. Synergistic effects can beobserved with the carnosine complex.

[0024] Typical concentrations for these compatible additives are about0.1% to about 5.0% by weight of antioxidants, about 0.1% to about 10% byweight of anti-inflammatory, about 0.1% to about 10% by weight of skinpenetrating agents, about 0.1% to about 5% by weight of neutralizingagents of reactive radicals, about 0.1% to about 2% by weight ofanti-inflammatory agents, about 1% to about 50% by weight of each ofcell stimulants, growth promoting and bio-activating agents, and immunesystem enhancers. The carnosine compositions and the carnosine complexescan be employed in topically applied cosmetic compositions such as thoseapplied to the skin in daytime, at nighttime, to tone the skin, to washskin surfaces such as the face as well as periorbitally around the eyes.

[0025] Water, adjusted to a pH of about 7.5 to about 9.5, preferablyabout 8.0 to about 8.5, is a desirable vehicle for carnosine and otheractive components. Carnosine and other ingredients, if present, aredispersed in the alkaline aqueous vehicle under ambient conditions. Thevehicle may desirably also contain a humectant such as glycerine orsorbitol, typically in amount of about 1% to about 10% by weight. Thevehicle may also be an emulsion with a lipid component such as liquidparaffin, squalene, glycerol distearate and the like present in atypical amount of about 0.001% to about 50% by weight. The entirecomposition is readily prepared free of allergenic components. Whencarnosine or derivative thereof is present with water only thecomposition is a suspension

[0026] Carnosine is particularly effective in preventing and reducingwrinkling of skin and may be effective in removing wrinkles as well, dueto its excellent anti-glycation properties and ability to breakcrosslinks associated with wrinkled skin or to prevent them fromforming. The effectiveness of carnosine as a wrinkle care material isquite unique to it in comparison to other amino acids including simpleamino acids such as alanine and histidine and polypeptides includingdipeptides such as glycylglycine and the like.

[0027] Carnosine and the various other ingredients described above, thatmay be present together with other additives that provide nutrients tothe skin and for other purposes well known in the art, may be presentfor treating epidermis (surface) and dermis (deep) (layers such as thosedescribed for wrinkle-care products in U.S. Pat. No. 5,939,078 toFujimura et al. the disclosure of which is incorporated herein byreference.

[0028] Such incorporated other additives typically may comprise about0.001 to about 50% by weight of the cosmetic composition and mayinclude:

[0029] humectants, ultra oily components, such as hydrocarbons, esters,high carbon fatty acids (e.g. stearic), high carbon alcohols (e.g.stearyl) and sphingosine derivatives from natural sources, typically inamount of about 0.005 to about 30% by weight; sterols, typically inamount of about 0.005 to about 30% by weight;

[0030] surfactants, especially nonionic surfactants, typically in amountof about 0.005 to about 30% by weight; water-soluble polyhydricalcohols, typically in amount of about 0.1 to about 25% by weight;powders, typically in amount of about 0.001 to about 50% by weight;

[0031] silicones, typically in amount of about 0.005 to about 30%; aswell as minor amounts of non-deteriorating salts, thickeners, pHregulators, violet absorbers, coloring agents, medicinal components,perfumes and the like. Other additives are included in the illustrativeexamples below.

[0032] The invention is illustrated by the following illustrativeexamples. All amounts and ranges are by in parts by weight unlessotherwise indicated.

EXAMPLE 1

[0033] The anti-wrinkling composition set forth below is prepared bydispersing the several ingredients with stirring in the alkaline aqueousvehicle. Component Parts L-carnosine 25 Gamma-tocotreniol  5 Licoriceroot  1 Embilica  1 L-theanine 10 Glutathione 10 L-tyrosine  5L-acetylcysteine  2 Clostrum  1 Lactoferrin  1 Deionized water (bufferedto Q.S. to 100 pH 8.0 (buffered to with aminometylpropanol,triethanolamine, monoisopropanolamine and dimethylmonoethanolamine)

[0034] The above composition is topically applied to skin It iseffective to reverse ageing by reducing wrinkles on wrinkled skin whenapplied to the skin in a regimen twice a day after for two weeks.

[0035] The pH of the above composition may be varied using alkalinewater and varying the pH with the buffer in the range of 7.5 to 9.5.

EXAMPLE 2

[0036] The composition of Example 1 is prepared except thatN-acetylcarnosine replaces carnosine. Again, skin wrinkling is reduced.

EXAMPLE 3

[0037] A suspension containing 35 parts of carnosine in water bufferedat pH 7.5 to 8.0 is prepared and is effective to reduce skin wrinkles.

EXAMPLE 4

[0038] The following compositions (A-G) are applied during daytime hoursfor anti-wrinkling ffectiveness and include age-reversal complexingredients. Parts Ingredient 4A 4B 4C 4D Phosphatidyl Choline 0.20 0.502.00 0.20 Sodium Hyaluronate 0.50 0.10 0.10 0.10 Sugarcane Extract 0.100.10 0.10 0.10 EDTA-Na₂* 0.10 — — 0.10 Zinc Sulfate — — — 0.10 NaCarboxymethyl 0.10 — — — Betaglucan Xanthan Gum — — — — Panthenol 0.05 —— — Et-hexyl 7.00 7.00 7.00 7.00 Methoxycinnamate Benzophenone-3 6.006.00 6.00 6.00 Isopropyl Palmitate — — — 5.00 Cetearyl Alcohol/ — — —3.00 ceteaarth-20 Cetearyl Alcohol 50/50 3.75 3.75 3.75 0.50 GlycerylStearate/ — — — 1.00 PEG* 100 Stearate Cetyl Esters 3.75 3.75 3.75 —Sorbitan Stearate 1.50 1.50 1.50 — Tetradecyl Ascorbate 0.10 0.25 0.500.10 Mg Boroascorbate-VDF — — 0.25 0.20 Vitamin A Palmitate 0.05 0.10 —— Tocotrienol R40 0.10 0.20 0.50 0.10 (Vitamin E) Polysorbate 60 1.501.50 1.50 — Ubiquinone 0.01 — — — (Ubidecarenone) Super Oxide Dismutase0.05 — — — PEG*-40 Castor Oil 0.25 1.50 1.50 — Squalane 1.00 — — —Benzyl Alcohol 1.50 1.50 1.50 — Phenoxyethanol & Me, — — — 0.25 Et, But,Pro & Isobut Parabens Rosemary Extract — 0.20 0.50 0.20 L-Carnosine15.00 15.00 15.00 15.00 L-Theanine 2.50 2.50 — — Green Tea Extract-VDF0.05 — — — Grape Seed Extract-VDF 0.05 — — — Vitamin K (powder) 0.050.10 0.10 0.10 Colostrum (powder) 0.05 0.05 0.05 0.05 Emblica (powder)0.10 0.05 0.05 0.05 Licorice (powder) — — — — Mn Gluconate.2 H₂O 0.050.05 0.05 0.05 N-Acetyl L-Cysteine — 0.01 0.01 0.01 Dimethylethanolamine0.10 0.15 0.15 0.15 Bergamot Oil — 0.001 0.001 0.001 Water (@ pH 8.0) q.s. 100 100 100 100 Parts Ingredient 4E 4F 4G Soy Lecithin — — 0.30Phosphatidyl Choline 0.10 0.10 0.20 Sodium Hyaluronate 4.00 4.00 4.00EDTA-Na₂ 0.10 0.10 0.10 Zinc Sulfate — — 0.10 Na CarboxymethylBetaglucan — — 0.20 Xanthan Gum — — 0.10 Et-hexyl Methoxycinnamate 7.007.00 7.50 Benzophenone-3 6.00 6.00 6.00 Isopropyl Palmitate 5.00 5.00 —Cetearyl Alcohol/ceteaarth-20 3.00 3.00 — Cetearyl Alcohol 50/50 0.500.50 3.75 Glyceryl Stearate/ 1.00 1.00 — PEG 100 Stearate Cetyl Esters —— 3.75 Sorbitan Stearate — — 1.50 Tetradecyl Ascorbate 0.25 0.25 0.10 MgBoroascorbate-VDF 0.20 0.20 — Vitamin A Palmitate — — 0.10 TocotrienolR40 0.50 0.50 0.10 Polysorbate 60 — — 1.50 Squalane — — 0.20 BenzylAlcohol — — 1.50 Phenoxyethanol & Me, Et, But, 0.30 0.30 — Pro & IsobutParabens Rosemary Extract 0.30 0.30 — L-Carnosine 15.00 5.00 15.00L-Theanine — — 2.50 Vitamin K (powder) 0.10 0.10 — Colostrum (powder)0.05 0.05 0.30 Emblica (powder) 0.05 0.05 0.10 Licorice (powder) — —0.10 Manganese Gluconate.2 H₂O 0.05 0.05 — N-Acetyl L-Cysteine 0.01 — —Dimethylethanolamine 0.15 0.15 0.40 Bergamot Oil 0.001 0.001 — Water (@pH 8.0) q.s. 100 100 100

[0039] An aqueous pre-mix is prepared using Wat r, Phosphatidyl Cholineand Sodium Hyaluronate as its base. Normally this aqu ous pre-mix isheated to approximately 60° C. to facilitate the dissolution ofPhosphatidyl Choline. Other components of this premix-Soy Lecithin,Sugarcane Extract, EDTA-Na₂, Zinc Sulfate, Na Carboxymethyl Betaglucan,Xanthan Gum, Emblica and Panthenol—are added in different combinationsto vary the characteristics of the aqueous phase and final product.

[0040] A non-aqueous pre-mix is also prepared with Et-hexylMethoxycinnamate, Benzophenone-3, Cetearyl Alcohol 50/50,Tetrahexyldecyl Ascorbate and Tocotrienol R40 as its base. Normally thisnon-aqueous pre-mix is heated until all components are melted anddispersed throughout the premix (˜60° C). Isopropyl Palmitate, CetylAlcohol/Ceteareth-20, Glyceryl Stearate/PEG 100 Stearate, Cetyl Esters,Sorbitan Stearate, Vitamin A Palmitate, Squalane, Mg Boroascorbate,Polysorbate 60, Ubiquinone (Ubidecarenone). Super Oxide Dismutase andPEG40 Castor Oil are added in different combinations to vary thecharacteristics of the non-aqueous phase and final product.

[0041] After these two pre-mixes are prepared and heated, they arecombined and the resulting mixture is stirred in order to achievecomplete dispersion. It is then allowed to cool. Either Benzyl Alcoholor Phenoxyethanol & Me, Et, But. Pro & Isobut Parabens, used as apreservative system, is added to the cooling dispersion before theaddition of L-Carnosine. To the resulting cooling dispersion, Vitamin K(powder), Colostrum (powder), Emblica (powder) and ManganeseGluconate•2H₂O are added in various combinations, as isDimethylethanolamine, that is DMEA (to adjust the pH), along with theother remaining chosen ingredients (L-Theanine, Licorice Powder, GreenTea Extract—VDF, Grape Seed Extract—VDF, N-Acetyl L-Cysteine, LavenderOil Fragrance and Bergamot Oil).

[0042] Upon reaching room temperature, the resulting cream is packaged

[0043] It is desirably applied one or two times a day over a two weekperiod.

EXAMPLE 5

[0044] The following compositions (A-C) are applied during nighttimehours for anti-wrinkling effectiveness and include age-reversal complexingredients (A and B are the most active; C is a simpler formula). PartsIngredient 5A 5B 5C Sodium Hyaluronate 0.10 — — HydrolyzedGlycosaminoglycans 0.10 — — Sugarcane Extract 0.05 — — EDTA-Na₂ 0.100.10 0.10 Phoshatidyl Choline — 4.00 — Na carboxymethyl Betaglucan 0.05— 0.05 Isopropyl Palmitate 6.25 6.25 6.25 Rice Bran Oil — 1.00 —Cetearyl Alcohol/Ceteareth-₂₀ mix 3.75 3.75 3.75 Cetearyl Alcohol 50/500.60 0.60 0.60 Glyceryl Stearate/PEG 100 Stearate 1.25 1.25 1.25Tetrahexyldecyl Ascorbate 0.10 0.10 0.50 Vitamin A Palmitate 0.05 0.100.01 Butylated Hydroxytoluene 0.30 — 0.30 PEG-40 Hydrogenated Castor Oil0.25 0.25 1.00 Tocotrienol R40 (Vitamin E) 0.05 0.10 — Ubiquinone(Ubidecarenone) 0.05 — — Super Oxide Dismutase 0.05 0.05 — MgBoroascorbate (VDF) — 0.10 — Squalane 1.00 — — Phenoxyethanol & Me, Et,but, 0.20 0.20 0.20 Pro. & Isobut Parabens L-Carnosine 15.00 15.00 15.00L-Theanine 1.00 — 1.00 Green Tea Extract - VDF 0.05 0.05 — Aloe Vera(200:1) Extract (Powder) 0.05 — — Colostrum (powder) 0.05 0.05 — Emblica(powder) 0.10 0.10 — Manganese Gluconate.2 H2O 0.05 0.05 —Dimethylethanolamine 0.10 0.20 0.20 Bergamot Oil — — 0.01 Water @ pH 8.0q.s. 100 100 100

[0045] An aqueous pre-mix is prepared using Water and EDTA-Na₂ (NaCarboxymethyl Betaglucan, Phosphatidyl Choline, Sugarcane Extract,Sodium Hyaluronate and Hydrolyzed Glycosaminoglycans are added indifferent combinations during the heating phase.) This aqueous pre-mixis heated to approximately 60° C.

[0046] A non-aqueous pre-mix is also prepared using Isopropyl Palmitate,Cetearyl Alcohol/Ceteareth-₂₀, Cetearyl Alcohol 50150, GlycerylStearate/PEG 100 Stearate, T trahexyldecyl Ascorbate, Vitamin APalmitate and PEG-40 Hydrogenated Castor Oil as its base. Normally thisnon-aqueous pre-mix was heated until all components are m ted anddispersed throughout the pre-mix (at approximately 60° C). For the‘active’ non-aqueous premix, Squalane, Tocotrienol R40, Ubiquinone(Ubidecarenone), Super Oxide Dismutase, Butylated Hydroxytoluene andPEG40 Castor Oil (hydrogenated) are also added in various combinationsto the mixture.

[0047] After the aqueous and non-aqueous pre-mixes are prepared andheated, they are combined and the resulting mixture is stirred in orderto achieve complete dispersion. It is then allowed to cool.Phenoxyethanol & Me, Et, But, Pro & Isobut Parabens, used as a presrvative system, is added to the cooling dispersion before the additionof L-Carnosine and L-Theanine. Dimethylethanolamine (DMEA) is added toadjust the pH in both dispersions, although it is added after the otherremaining ingredients in the formulation (Green Tea Extract—VDF, AloeVera (200:1) Extract (Powder), Colostrum (powder), Emblica (powder), MgBoroascorbate (VDF) and Manganese Gluconate-2H20 in varyingcombinations). As a final step in the cooling ‘simple’ dispersionBergamot Oil is added.

[0048] Upon reaching room temperature, the resulting cream was packaged.It is desirably applied at night over a two week period.

EXAMPLE 6

[0049] The following compositions (A & B) are applied to tone the skinwhile providing anti-wrinkling effectiveness and included age-reversalcomplex ingredients. Parts Ingredient A B L-Carnosine 1.00 5.00L-Theanine 0.10 — Allantoin 0.05 0.10 Na PCA (Pyrocarboxylic Acid Na)0.10 0.25 Sorbitol (70%) 0.25 0.10 Aloe Vera (200:1) Powder 0.05 0.01Actiphyte of Witch Hazel 1.00 0.50 Actiphyte of Echinacea 1.00 0.50Actiphyte of Chamomile 1.00 0.50 EDTA-Na₂ 0.10 0.05 PEG-60 Castor Oil(hydr) 0.05 0.05 Bergamot Oil 0.05 0.01 Phenoxyethanol & Me, Et, Bu,Pro, — 0.10 & Isobut Parabens Pentylene Glycol — 0.10 Water (@ pH 8.0)q.s. 100 100

[0050] To the water, which is at room temperature, is added each of theother ingredients, one at a time, allowing for each to dissolve beforethe addition of the next. The additions are done while the aqueoussolution is being stirred.

[0051] After sufficient stirring to insure a uniform solution, theresulting toner is packaged. It is desirably applied one to two times aday over a two week period.

EXAMPLE 7

[0052] The following compostion is applied to the the face as a softfacial wash with anti-wrinkling effectiveness and includes age-reversalcomplex ingredients. Ingredient Parts Mackadet EQ-76 52.00 (Deionizedwater & PEG 80 Sorbitan Laurate, Cocamidopropyl Na Tridecadeth Sulfate,Na betaine Lauroamphoacetate PEG 150 Distearate, Na laureth-13Carboxylate PEG 150 Distearate, Citric Acid, Quaternium 15 & Na₄ EDTA)L-Carnosine 5.00 Na PCA (Pyrocarboxylic Acid Na) 0.10 Jojoba Oil (BostonJojoba) 0.01 Sugarcane Extract 0.25 Dimethanolethanolamine 0.15 Pomelo7713 (Fragrance) 0.15 DMDM Hydantoin 0.15 Water q.s. 100

[0053] The water is heated to ˜45° C. and the following ingredients areadded one at a time to the water, with stirring, allowing enough timefor dissolution of the previous ingredient: Mackadet EQ-76, L-Carnosine,Na PCA, Jojoba Oil (Boston Jojoba) and Sugarcane Extract. The aqueoussolution is cooled to room temperature at which the remainingingredients (Dimethylethanolamine, that is DMAE), Pomelo 7713 and DMDMHydantoin) are added with stirring, allowing enough time for dissolutionof the previous ingredient.

[0054] After sufficient stirring to insure a uniform solution, theresulting skin wash is packaged. It is desirably applied to wash theface once a day over a period of two weeks.

EXAMPLE 8

[0055] The following compositions (A & B) are applied periorbitablyaround the eyes for their anti-wrinkling and age-reversal effects,preferably once daily over a two week period. Parts Ingredient 8A 8BPhosphatidyl Choline 0.20 1.00 Sodium Hyaluronate 4.00 0.50 EDTA-Na20.10 0.10 Panthenol 0.10 0.10 Mg Boroascorbate-VDF — 0.25 EthylhexylMethoxycinnamate 5.00 5.00 Cetearyl Alcohol 50/50 3.75 3.75 Cetyl Esters3.75 3.75 Sorbitan Stearate 1.50 1.50 Polysorbate 60 1.50 1.50 Squalane0.10 — Ceramide-III 0.10 0.10 Tocotrienol R40 (Vitamin E) 0.10 0.20Ubiquinone (Ubidecarenone) 0.10 0.10 Super Oxide Dismutase 0.10 0.10Vitamin A Palmitate 0.05 0.05 Tetrahexyldecyl Ascorbate 0.10 0.10 BenzylAlcohol 1.50 1.50 L-Carnosine 10.00 10.00 L-Theanine 0.50 0.50 Vitamin K(powder) 0.05 0.05 Green Tea Extract - VDF 0.05 0.05 Colostrum (powder)0.10 0.10 Cucumber Extract 0.10 0.10 Dimethylethanolamine 0.15 0.20Water (@ pH 8.0) q.s. 100 100

[0056] An aqueous pre-mix is prepared using Water, Phosphatidyl Choline,Sodium Hyaluronate, EDTA-Na₂ and Panthenol as its base. Normally thisaqueous pre-mix is heated to at approximately 60° C. to facilitate thedissolution of Phosphatidyl Choline. Mg Boroascorbate is added to one ofthe formulations but not to the other.

[0057] A non-aqueous pre-mix was also prepared with EthylhexylMethoxycinnamate, Cetearyl Alcohol 50/50, Cetyl Esters, SorbitanStearate, Polysorbate 60, Ceramide III, Tocotrienol R40, Ubiquinone(Ubidecarenone), Super Oxide Dismutase, Vitamin A Palmitate andTetrahexyldecyl Ascorbate as its base. This non-aqueous pre-mix isheated until all components are melted and dispersed throughout thepre-mix (at approximately 600 C). Squalane is added to the pre-mixnon-aqueous formulation for the product not containing Mg Boroascorbatein its aqueous pre-mix.

[0058] After these two pre-mixes are prepared, heated and combined andthe resulting mixture is stirred in order to achieve completedispersion. It is then allowed to cool. Benzyl Alcohol, used as apreservative system, is added to the cooling dispersion before theaddition of L-Carnosine and L-Theanine. To the resulting coolingdispersion, Vitamin K (powder), Colostrum (powder), Green TeaExtract-VDF and Cucumber are added. The last step is the addition ofDimethylethanolamine (DMEA) to adjust the pH.

[0059] Upon reaching room temperature, the resulting cream is packaged.

[0060] It will be apparent that modifications and variations within thescope of the present invention may be prepared and provided.

1. An alkaline cosmetic composition having a pH of about 7.5 to about9.5, said composition being effective against skin wrinkling, saidcomposition comprising carnosine dispensed in an alkaline cosmeticallyacceptable vehicle in an effective antiwrinkle amount of up to about 50%by weight of carnosine, derivatives thereof and mixtures of carnosineand derivatives thereof.
 2. The alkaline cosmetic composition claimed inclaim 1 wherein the amount of carnosine, derivative thereof or mixtureof carnosine and derivative thereof is about 0.5% to about 50% byweight.
 3. The alkaline cosmetic composition claimed in claim 2 whereincarnosine, derivative thereof or mixture of carnosine and derivativethereof is present in amount of about 1.5% to about 30% by weight. 4.The alkaline cosmetic composition claimed in claim 2 wherein carnosine,derivative thereof or mixture of carnosine and derivative thereof ispresent in amount of about 10% to about 20% by weight.
 5. The alkalinecosmetic composition claimed in claim 1 wherein carnosine is present. 6.The alkaline cosmetic composition claimed in claim 1 wherein N-acetylcarnosine is present.
 7. The alkaline cosmetic composition claimed ofclaim 1 wherein said carnosine, derivatives of carnosine and mixtures ofcarnosine and derivatives of carnosine are present in a complex with atleast one of an antioxidant agent, an anti-inflammatory agent, a skinpenetration agent, a neutralizing agent of reactive radicals, ananti-inflammatory agent, a cell stimulant, a growth promoting andbio-activating agent, and an immune system enhancer.
 8. The alkalinecosmetic composition claimed in claim 1 wherein said cosmeticcomposition is a composition for application to the skin as a daytimecomposition, a nighttime composition, a skin toning composition, afacial wash composition, or as a periorbital composition applied aroundthe eyes.